GECRY SCHOOL #1
From Gene to Protein Crystal
This school was thought for all those who want to approach crystallography as a technique for structural biology studies and have limited or no experience in this field. The main target of the School was to provide the most important and frequently used biological and crystallographic tools needed to get protein crystal structures starting from gene information.
The School was a full immersion hands-on experience alternating theoretical information (condensed in a few lessons) and at least 4 hours per day be dedicated to experiments in the lab for protein expression and crystal preparation
The first edition of the School was held at ELETTRA Synchrotron (Basovizza, TS)
Speakers
Martino Bolognesi
Paola Storici
Giusy Tassone
Doriano Lamba
Silvia Onesti
Barbara Giabbai
Marta Semrau
Cecilia Pozzi
Marco Mazzorana
Rocco Caliandro
Benny Danilo Belviso
Local committee
Silvia Onesti
Paola Storici
Barbara Giabbai
Marta Semrau
Annie Heroux
TOPICS OF LECTUREs
Bio-Crystallography: past, present and future
Cloning and site-directed mutagenesis
Protein expression in eukaryotic and prokaryotic systems
Co-expression systems
Chromatographic techniques
Protein Purification strategies
Protein quality control for structural biology
Crystallization: how it works
Crystallization techniques
Membrane protein crystallization
Crystal handling
Bioinformatics analysis
Laboratory practicals
Primer design
Cloning
Protein expression in E. coli
Protein purificaition by Affinity chromatography
Size-exclusion chromatography
SDS-PAGE analysis
Solubility test for crystallization
Vapour diffusion crystallization
Crystal seding
SPONSORS
The GeCry School #1 was generously sponsored by:
